CNS immune signaling contributes to deleterious opioid effects including hyperalgesia tolerance reward and dependence/withdrawal. (M3G) both known TLR4 agonists. We demonstrate that adult rat CNS endothelial cells express functional TLR4. M3G activated NF��B increased tumor necrosis factor-�� (TNF��) and cyclooxygenase-2 (COX2) mRNAs and released prostaglandin E2 from these cells. (-)-Morphine-induced upregulation of TNF�� mRNA and… Continue reading CNS immune signaling contributes to deleterious opioid effects including hyperalgesia tolerance