High-throughput short-read technologies have revolutionized DNA sequencing by drastically reducing the cost per base of sequencing information. DNA sequencing technology has benefited from tremendous progress over the past several years, with many platforms routinely producing >109 nucleotides (nt) of data during a single run [1]. Current generation high-throughput sequencers require a library of amplicons from… Continue reading High-throughput short-read technologies have revolutionized DNA sequencing by drastically reducing the