Supplementary Materials [Supplementary Data] gkp812_index. These were after that immortalized by co-transfection having 808118-40-3 a SV40 build and a puromycin manifestation vector at Passing 2 and chosen in 2 g/ml puromycin. The immortalized Rabbit polyclonal to HSD3B7 MEFs had been cultured in DMEM supplemented with 10% Fetal Bovine Serum and 2 mM l-Glutamine. To stimulate… Continue reading Supplementary Materials [Supplementary Data] gkp812_index. These were after that immortalized by