Supplementary Materialsviruses-10-00557-s001. at area temperatures for 2 h, accompanied by incubation with primary antibodies at 4 C overnight. The PVDF membrane was after that cleaned with TBST and additional incubated with horseradish peroxidase-conjugated second antibody. The membranes had been cleaned with TBST, as well as the immunoblots had been developed with improved chemiluminescence recognition (ECL,… Continue reading Supplementary Materialsviruses-10-00557-s001. at area temperatures for 2 h, accompanied by incubation