Vault is a 12. at the end of the vault caps (Kong (1999 ?). The protein concentration was identified using a BCA Protein Assay Kit (Pierce). The vault purified from a CsCl density-gradient portion was dialyzed against buffer comprising 50?mTris pH 7.5, 75?mNaCl and 1.5?mMgCl2 and then concentrated to 10C80?mg?ml?1 in an ultrafiltration cell (Orbital… Continue reading Vault is a 12. at the end of the vault caps