Supplementary MaterialsSupplementary Information 41598_2019_48130_MOESM1_ESM. gene expression, the CRISPR/Cas9 was utilized by us knock-in and CRISPR/dCas9-Tet1 systems. Using both of these techniques, we induced site-specific demethylation in the Oct4 promoter and verified the up-regulation of Oct4 manifestation. Furthermore, we verified how the synergistic aftereffect of DNA demethylation and additional epigenetic regulations improved the manifestation of Oct4… Continue reading Supplementary MaterialsSupplementary Information 41598_2019_48130_MOESM1_ESM. gene expression, the CRISPR/Cas9 was utilized by